By 2050 we?ll need to feed two billion more people. How can we achieve that without overwhelming the planet??
The question raised by 2009 demands quick action. Human life depends on plants, seeds, which feeds us, our
livestock or fulfils our biofuel needs. This demands investment efforts in understanding the basis of reproductive
traits and its relationship with phenotypic plasticity. Knowing the fundamental molecular processes that finely
regulate plant reproduction, will allow increasing crop productivity and high quality seed production in a sustainable
manner, safeguarding seed security of farming communities. This project aims to provide new insights into the
role of Arabinogalactan Proteins (AGPs), signalling molecules crucial for plant reproduction, and understanding
its transcriptional regulation by Histone Deacetylase 19 (HDA19) and 7 (HDA7). Uncovering its functions, targets,
receptors, putative signalling pathways, epigenetic and signalling control during reproduction in Arabidopsis thaliana
through a combination of genetic, proteomic and transcriptomic approaches is this project main goal. This proposal,
which addresses critical stages of reproductive development in Arabidopsis, integrates several approaches to
comprehensively investigate sexual reproduction in plants with the aim of applying the novel knowledge generated to
improve crop production, both quantitatively and qualitatively.
Publicações :
*Costa M, Pereira AM, Pinto SC, Silva J, Pereira LG, Coimbra S. 2019. In silico and expression analyses of fasciclin‑like arabinogalactan proteins reveal functional conservation during embryo and seed development. Plant Reproduction. URL: https://doi.org/10.1007/s00497-019-00376-7.
*Pereira AM, Coimbra S. 2019. Advances in plant reproduction: from gametes to seeds. Journal of Experimental Botany. 70(11): 2933-2936. URL: https://academic.oup.com/jxb/article/70/11/2933/5509873
*Lopes AL, Moreira D, Ferreira MJ, Pereira AM, Coimbra S. 2019. There are secrets along the pollen tube pathway, still in need to be discovered. Journal of Experimental Botany. 70(11): 2979-2992.URL: https://repositorio-aberto.up.pt/bitstream/10216/127892/2/407997.pdf
*Costa, M, Silva, J, and Coimbra, S. 2020. Arabidopsis thaliana pollen tube culture for multi-omics studies. Pollen and Pollen Tube Methods, In lab protocol series Methods in Molecular Biology. Ed. Anja Geitman. Springer Nature. https://experiments.springernature.com/articles/10.1007/978-1-0716-0672-8_10
*Costa, ML, Solís M-T, Testillano, PS, and Coimbra, S. 2020. In situ/Subcellular localization of arabinogalactan protein expression by fluorescent in situ hybridization, FISH. In The plant cell wall: Methods and protocols. Ed. Zoe Popper. 2nd edition
*Moreira D, Pereira AM, Lopes AL, Coimbra S. 2020. The best CRISPR/Cas9 versus RNA interference approaches for Arabinogalactan proteins' study. Molecular Biology Reports. DOI: 10.1007/s11033-020-05258-0
*Pereira, A. M., Moreira, D., Coimbra, S., & Masiero, S. 2021. Paving the Way for Fertilization: The Role of the Transmitting Tract. International journal of molecular sciences, 22(5), 2603. https://doi.org/10.3390/ijms22052603. IF: 5.923
*Rojek J., Tucker M. R., Pinto S. C., Rychlowski M., Lichocka M., Soukupova H., Nowakowska J., Bohdanowicz J., Surmacz G., Gutkowska M. 2021. Rab-dependent vesicular traffic affects female gametophyte development in Arabidopsis. Journal of Experimental Botany, 72 (2), 320-340, https://doi.org/10.1093/jxb/eraa430. I
*Ferraz, R.A.C., Lopes, A.L.G., da Silva, J.A.F., Moreira D., Ferreira, M.J., Coimbra S. 2021. DNA-protein interaction studies: a historical and comparative analysis. Plant Methods 17, 82. https://doi.org/10.1186/s13007-021-00780-z. IF: 5.14
*Costa M., Pereira A.M., Coimbra S. 2021. Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues. J Vis Exp. 27(168). doi: 10.3791/61034. PMID: 33720133.
*Moreira E, Coimbra S, Melo P. 2022. Glutamine synthetase: an unlikely case of functional redundancy in Arabidopsis thaliana. Plant Biology doi:10.1111/plb.13408
*Kaur D, Moreira D, Coimbra S, Showalter AM. 2022. Hydroxyproline-O-Galactosyltransferases Synthesizing Type II Arabinogalactans Are Essential for Male Gametophytic Development in Arabidopsis. Front. Plant Sci. 13:935413. doi: 10.3389/fpls.2022.935413
* Figueiredo R, Lopes A, Pereira AM, Moreira D, Silva J, Ferreira MJ, Pereira LG, Coimbra S. 2022. The biology of arabinogalactan proteins in plant abiotic stress and reproduction. In The plant cell wall - From Robert Hooke's cells to polymer atomic structure. CRC Press (Taylor & Francis Group). |
